recombinant mouse grn protein  (Sino Biological)

Journal: Scientific Reports

Article Title: Imbalance between TNFα and progranulin contributes to memory impairment and anxiety in sleep-deprived mice

doi: 10.1038/srep43594

Figure Lengend Snippet: ( A ) Schematic of experimental procedures. ( B ) Sample of PGRN intraventricular injection (ICV) site. ( C ) Western-blot samples of PGRN and TNFα and band intensities after thalidomide (Tha) treatment in S-DEP mice. ( D ) Preference index of control, S-DEP, PGRN (ICV, 5 ng), and Tha (ip, 25 mg/kg) treated mice in novel object recognition test. ( E ) Locomotors activities and time spent in the center area of control, S-DEP, PGRN (ICV, 5 ng), and Tha (ip, 25 mg/kg) treated mice in open field test. n = 6 mice per group; one-way ANOVA with LSD test, * p < 0.05, ** p < 0.01 versus control mice, # p < 0.05 versus saline treated S-DEP mice.

Article Snippet: Mice received intracerebroventricular (ICV) injections of recombinant PGRN (5 ng/5 μl in 0.9% saline; Sino Biological Inc., Beijing, China), TNFα (10 ng/5 μl in 0.9% saline; Sino Biological Inc.), or 5 μl 0.9% saline by a polyethylene tube to a 10 μL Hamilton syringe at a rate of 1 μl/min 3 days before sleep deprivation.

Techniques: Injection, Western Blot

Journal: Scientific Reports

Article Title: Imbalance between TNFα and progranulin contributes to memory impairment and anxiety in sleep-deprived mice

doi: 10.1038/srep43594

Figure Lengend Snippet: ( A ) Golgi-Cox-staining of CA1 pyramidal neurons for spine counting. ( B ) Left: Representative images of basilar dendrites from control, saline (ICV, 5 μl) treated S-DEP, TNFα treated (ICV, 10 ng/5 μl) S-DEP, and PGRN treated (ICV, 5 ng/5 μl) S-DEP mice. Right: Summary of spine counts from basilar dendrites. n = 40 neurons/4 mice per group; two-way ANOVA with LSD test, ** p < 0.01 versus control mice, ## p < 0.01 versus saline treated S-DEP mice.

Article Snippet: Mice received intracerebroventricular (ICV) injections of recombinant PGRN (5 ng/5 μl in 0.9% saline; Sino Biological Inc., Beijing, China), TNFα (10 ng/5 μl in 0.9% saline; Sino Biological Inc.), or 5 μl 0.9% saline by a polyethylene tube to a 10 μL Hamilton syringe at a rate of 1 μl/min 3 days before sleep deprivation.

Techniques: Staining

Journal: Scientific Reports

Article Title: Imbalance between TNFα and progranulin contributes to memory impairment and anxiety in sleep-deprived mice

doi: 10.1038/srep43594

Figure Lengend Snippet: ( A ) Representative micrograph of double staining to identify newborn neurons (EdU/red) in the dentate gyrus of the hippocampus. Left: scale bar = 100 μm; Right: scale bar = 50 μm. ( B ) Number of EdU positive neurons in the dentate gyrus subgranular zone from control, saline (ICV, 5 μl) treated, TNFα treated (ICV, 10 ng/5 μl), and PGRN treated (ICV, 5 ng/5 μl) S-DEP mice. n = 3 mice per group; one-way ANOVA with LSD test, ** p < 0.01 versus saline treated control mice, ## p < 0.01 versus saline treated S-DEP mice.

Article Snippet: Mice received intracerebroventricular (ICV) injections of recombinant PGRN (5 ng/5 μl in 0.9% saline; Sino Biological Inc., Beijing, China), TNFα (10 ng/5 μl in 0.9% saline; Sino Biological Inc.), or 5 μl 0.9% saline by a polyethylene tube to a 10 μL Hamilton syringe at a rate of 1 μl/min 3 days before sleep deprivation.

Techniques: Double Staining